The preparation of ribonucleic acid from yeast, tobacco leaves and tobacco mosaic virus
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چکیده
منابع مشابه
Sequences of oligonucleotides prepared from tobacco mosaic virus ribonucleic acid.
Sequence anal,yses have been performed on 11 long-chainlength oligonucleotides isolated from RNase T, digests of tobacco mosaic virus ribonucleic acid. The chainlengths of these oligonucleotides range from 14-72 nucleotides. The structures of six oligonucleotides have been determined, including one that corresponds to a portion of the amino acid sequence in the coat protein. Partial sequences h...
متن کاملLocation of unique sequences in tobacco mosaic virus ribonucleic acid.
Tobacco mosaic virus has been partially stripped of its protein coat by sodium dodecyl sulfate. The nucleic acid exposed was digested with T1 ribonuclease and the products were examined for the presence of three oligonucleotides of unique sequence. The results indicate the location of two of the unique oligomers to be near the 5’-OH-linked end and the third to be near the middle of tobacco mosa...
متن کاملInduction of UDP-Glucose:Salicylic Acid Glucosyltransferase Activity in Tobacco Mosaic Virus-Inoculated Tobacco (Nicotiana tabacum) Leaves.
Salicylic acid (SA) is a putative signal that activates plant resistance to pathogens. SA levels increase systemically following the hypersensitive response produced by tobacco mosaic virus (TMV) inoculation of tobacco (Nicotiana tabacum L. cv Xanthi-nc) leaves. The SA increase in the inoculated leaf coincided with the appearance of a [beta]-glucosidase-hydrolyzable SA conjugate identified as [...
متن کاملInfectivity of ribonucleic acid from tobacco mosaic virus containing 2-thiouracil.
By the mid 1950s, there was no doubt that the genetic material of all living cells is DNA. It was also known, however, that many viruses—animal, plant, and bacterial—do not contain DNA, but RNA, and that therefore RNA must also be able to replicate, function as a repository for genetic information, and also express it. In fact, Gierer and Schramm went one step further: they showed that tobacco ...
متن کاملCharacterization of double-stranded ribonucleic acid in tobacco leaves.
Double-stranded RNA was isolated from tobacco leaves and characterized in terms of base composition, density, and nuclease resistance. Although its role in the plant's physiology is not clear, evidence was adduced that it is the product of the RNA-dependent RNA polymerase previously shown to occur in this and other plants. The fact that twice as much fully than partially double-stranded RNA app...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1955
ISSN: 0306-3283
DOI: 10.1042/bj0600046